Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

- Overview
  - The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
    
    Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase)/lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
    
    Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Limit of Detection: (Malt β-glucanase) 4.3x10-4U/mL Or (Lichenase) 9.1x10-5U/mL
    
    Storage
    
    Short term stability: 2-8°C
    Long term stability: See individual component labels
    
    Stability
    
    > 2 years under recommended storage conditions
    
    * For research use only.
- Applications
  - Application
    
    Activity Assay
    
    Application Description
    
    Crude malt extracts, industrial enzyme preparations.
- Reference
  - 1.Prediction of potential malt extract and beer filterability using conventional and novel malt assays. Cornaggia, C., Evans, D. E., Draga, A., Mangan, D. & McCleary, B. V. (2019). Journal of Institute of Brewing, 125(3), 294-309.
    2.Novel approaches to the automated assay of β-glucanase and lichenase activity. Mangan, D., Liadova, A., Ivory, R. & McCleary, B. V. (2016). Carbohydrate Research, 435, 162-172.
    3.Ultrasound-assisted modification of enzyme and antioxidant activities, functional and rheological properties of oat and barley bran. Grgić, T., Pavišić, Z., Strmečki, N. M., Voučko, B., Mustač, N. Č., Ćurić, D., Le-Bail, A. & Novotni, D. (2023). Research Square, In Press

MSDS

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

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