Protein A/G Coated PCR Plates

Protein A/G Coated PCR Plates are made in clear polypropylene coated with different molecules for your needs. Their thin walls optimize the heat transfer from the block to the reaction solution. while the rim of the wells is designed to protect against cross-contamination, allowing a reliable seal with the sealing mats tailored to the plates.

· PCR plates are pre blocked with standard proteic blocking (ELISA Blocking)
· Recommended working volume of 50 to 100 μl
· Each coated plate is packed in a single barrier bag with desiccant

Surface:
Protein A/G coated surface on Protein A/G coated PCR plate binds to most IgG species (in particular binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin and to all subclasses human IgG), IgA, IgE, IgM and IgD. This product can be used in purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG.

Please contact us at for specific academic pricing.

Background

Protein A/G Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.
Protein A/G Coated Surface is a surface that binds to most IgG species (in particular binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin and to all subclasses human IgG), IgA, IgE, IgM and IgD.

Protein A/G includes four Fc binding domains from Protein A and two from Protein G making it a versatile tool. The binding dependency to pH of Protein A/G is lower than Protein A but has the additive properties of Protein A and G together.

Protein A/G binds to human IgG, IgA, IgM; it binds to all subclasses of mouse IgG excluding mouse IgA, IgM and serum albumin (see Table 1). When coated onto microplates, the Protein A/G can securely be used in purification and detection of mouse monoclonal IgG antibodies with no interference from IgA, IgM and serum albumin.

· Examples of applications for Protein A/G Coated surface
specific and sterically oriented bond of antibodies
highest specificity and capacity
retains antibody activity and orients antibody for maximum binding
generally not suitable for sandwich ELISA assays

· Protein A/G Coated Surfaces main features
Biomat Protein A/G Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Protein A/G Coated Products ensures:

Uniformity
The Protein A/G Coated Products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability
The Protein A/G Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Protein A/G Coated Microplates Specifications
Coating
Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli. Protein A/G is coated using 200 µl/well.

Binding capacity
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.

Protein A/G Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli. Protein A/G is coated using 100 µl/tube.