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Overview
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MAL-dPEG®12-acid, product number 10285, is a crosslinker that joins sulfhydryl groups to free amines through a single molecular weight polyethylene glycol (PEG) chain of discrete length. The maleimidopropyl moiety reacts with sulfhydryl groups via a Michael addition reaction. The propionic acid terminus of the molecule reacts directly with free amines using EDC or another carbodiimide to form amide bonds. Alternatively, activation of the propionic acid group with N-hydroxysuccinimide (NHS), 2,3,5,6-tetrafluorophenol (TFP), or some other acylating agent permits amide bond formation under mild, controlled conditions.
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