MAL-dPEG®₂-TFP ester

MAL-dPEG®2-TFP ester, product number 10549, is a crosslinking reagent that joins free amines to free thiols (sulfhydryl groups). The sulfhydryl groups react with a maleimido group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the 2,3,5,6-tetrafluorophenyl (TFP) ester of the terminal propionic acid group. The maleimido and TFP functional groups on the crosslinking compound sit at either end of a short, discrete polyethylene glycol (dPEG®) chain.

Crosslinking Reactions
One of the most popular, most useful crosslinking reactions in bioconjugate chemistry is the conjugation of free amines to free thiols. These reactions require heterobifunctional reagents that bridge the two groups. Typical crosslinkers are hydrophobic. Our dPEG® crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths.

With conventional hydrophobic crosslinking reagents, aggregation and precipitation of the conjugates occur frequently. These problems do not happen with our water-soluble, non-immunogenic dPEG® crosslinkers.

Please contact us at for specific academic pricing.