MAL-dPEG®₂-NHS ester

MAL-dPEG®2-NHS ester, product number 10266, is a crosslinking reagent that joins a sulfhydryl to a free amine. The sulfhydryl groups react with a maleimide group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a short, discrete-length polyethylene glycol chain (dPEG®).

One of the most popular, most useful crosslinking reactions in bioconjugate chemistry is the reaction that joins a sulfhydryl group to a free amine. These reactions require heterobifunctional reagents that bridge the two different reactive groups. Initially, such reagents were initially hydrophobic. Now, our dPEG® products are single molecular weight PEG compounds with discrete chain lengths. Our amphiphilic dPEG® products impart water solubility to conjugates containing them.

Consequently, the problems that accompany hydrophobic crosslinking reagents, such as aggregation and precipitation of biomolecular conjugates, do not exist with dPEG® crosslinkers.

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