Kanamycin Acid Sulfate, BP

- Overview
  - Kanamycin Acid Sulfate, BP is an aminoglycoside antibiotic often used to select for bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance. Kanamycin is very soluble in aqueous solution at 92.3 mg/mL.
    
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    Background
    
    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
- Properties
  - CAS Number
    
    64013-70-3
    
    Molecular Formula
    
    C18H36N4O11 · 2 H2SO4
    
    Molecular Weight
    
    680.65
    
    Appearance
    
    Colorless solid
    
    Solubility
    
    Freely soluble in water
    
    Other Properties
    
    Source: Streptomyces Kanamyceticus
    
    Storage
    
    2-8 °C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Kanamycin is a broad spectrum antibiotic; however, it is mostly used against aerobic Gram-negative bacteria.
    
    Microbiology Applications: Kanamycin Acid Sulfate is commonly used as a selective agent to select for resistant mammalian, fungal, or bacterial cells that contain the kanMX marker or other Kanamycin resistance genes. Kanamycin Acid Sulfate is typically used at a concentration of 50 µg/mL.
    Pryjma et al.used Kanamycin Sulfate (TOKU-E) to select for transformed kanamycin resistant Campylobacter jejuni cells in: "FdhTU-modulated formate dehydrogenase expression and electron donor availability enhance recovery of Campylobacter jejuni following host cell infection"
    
    Media Supplements
    Kanamycin can be used as a selective agent in several types of isolation media:
    Kanamycin Aesculin Azide Agar - Enterococci isolation in food
    Perfringens Agar - SFP and TSC selective supplements for the isolation of Clostridium perfringens
    
    Plant Biology Applications: Kanamycin is often used in the Agrobacterium mediated transformation while using the npt II gene as selection marker. Kaur and Bansal (2010) used kanamycin in combination with cefotaxime to control bacterial growth while transforming tomatoes.