Human transglutaminase 7

- Overview
  - His6-rhTG7 is based on the TGM7-gene on plasmid pCRII-hTGz cl.14 (isolated by Daniel Aeschlimann), corrected by the insertion of a C at position 1169. It is N-terminally fused to a hexahistidine-tag.
    
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    Zedira
- Properties
  - Name
    
    TG7, TGase 7
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 90 % (visually by SDS-PAGE)
    
    Formulation
    
    The Transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.
    
    Storage
    
    Store at ≤ -20°C. Store working aliquots at ≤ -20°C. Avoid repeated freezing and thawing. Delivery at ambient temperature is possible
    
    Note
    
    Intended for research use only, not for use in human, therapeutic or diagnostic applications.
    
    Molecular Weight
    
    81 kDa
    
    Class
    
    Transglutaminase 7
    
    Reconstitution
    
    Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage.
    
    Activity
    
    His6-rhTG7 is a Ca2+-dependent enzyme. Add 10 mM Ca2+ to activate His6-rhTG7.
- Applications
  - Application Description
    
    His6-rhTG7 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG7 may also be used for immunoprecipitation.
- Reference
  - Kuramoto at al., Arch: Biochem Biophys. 2013; 537138-43
    Fukui et al., FEBS J. 2013, 280:1420-9

Datasheet

MSDS

Human transglutaminase 7

More Details

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