HUMAN α/β TCR DNA MULTIPLEX Kit

The Human α/β TCR DNA Kit enables highly efficient amplification of rearranged T-cell receptor (TCR) alpha and beta genes using human genomic DNA as a template. The kit utilizes multiplex PCR with gene-specific primers targeting TCR joining (J) and variable (V) regions. All primers and reaction conditions are selected in a way that minimizes amplification bias and allows for accurate and sensitive TCR clonotypes diversity assessment.
The kit allows to start with DNA derived from 1,000 to 50,000 sorted/purified T cells, from peripheral blood leukocytes (PMBC), or from T cell-containing tissues (fresh-frozen or formalin-fixed paraffin-embedded).
The amplification of TCR alpha and TCR beta chains is performed simultaneously in the first PCR reaction. Second PCR amplification is used to introduce Illumina sample barcodes (Nextera Unique Dual Indexes) and oligonucleotides necessary for sequencing. The use of Unique Dual Indexes (UDI) significantly decreases cross-sample contamination resulting from “barcode hopping” effect. It is possible to use standard Nextera XT indexing primers however the level of cross-sample contamination will be high (up to 1%).
Obtained PCR products can be sequenced on any Illumina platform. Sequences allow reconstructing repertoire of alpha/beta T-cell receptors in a sample of interest.

MiXCR is a universal solution for fast and rigorous extraction and analysis of T-cell and B-cell receptor repertoire high-throughput sequencing data.

Please contact us at for specific academic pricing.