HRP Oligo Conjugation Kit (Single Label with Thiol Oligo) (Replacement)

This PerKit™ HRP−Oligo conjugation kit to prepare single-labeled HRP−Oligo conjugates with linkers and conjugation chemistry that retains the activity of HRP. The same configuration and chemistry have been tested for HRP-oligos with different oligo sequences at a few different diagnostic companies since 2011.

This HRP-Oligo Conjugation kit works with protected thiol-modified oligos (disulfide form). The customer supplies the thiol-modified oligos containing 3’ C6 disulfide linker or 5’ C6 disulfide linker, which are readily available from many commercial oligo suppliers. Using the kit components, the customer reduces the disulfide bond of the oligo, followed by reaction of the thiol-oligo with activated horseradish peroxidase (HRP) to generate the HRP-oligo conjugates. The Q-column purification step typically provides the resulting HRP-oligo at greater than 85% purity.

This kit is an upgraded and improved replacement kit with slightly different components and workflow as the first-generation kit (Cat# CM53402). This kit has multiple configurations for various lengths of oligos and can be used to conjugate 2 to 5 nano-mole (nmol) of oligo.

• Requirements for disulfide-oligo
Total amount: 2 to 5 nano-mole oligo content as measured by UV.
HPLC purified with disulfide-oligo content ≥90% by C18 HPLC.
≥5- bases (oligos longer than 60 bases may result in lower loading and high impurities).

• Key Features of this HRP-Oligo Conjugation Kit
High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity.
A single purification step affords around 85-90% of single labeled HRP-oligo conjugates if the quality of the oligo is at par.
Preparation can be done within a few hours.
Stable linkage with optimal spacer to ensure no interference between oligo binding and HRP activation.
All reagents included from preparation to purification.

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