HRP Oligo Conjugation Kit (Single Label with Amine Oligo) (Replacement)

This PerKit™ HRP−Oligo conjugation kit to prepare single-labeled HRP−Oligo conjugates with linkers and conjugation chemistry that retains the activity of HRP. The same configuration and chemistry have been tested for HRP-oligos with different oligo sequences at a few different diagnostic companies since 2011.

The kit is designed to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. Using the kit components, the user converts the amine-oligo to a thiol-oligo, followed by reaction of the thiol-oligo with activated HRP to generate the HRP-oligo conjugates. The total length of the linkage between the HRP and the amine-oligo is 29 atoms excluding the linker used for amine modification of the oligo. The Q-column purification step typically provides the resulting HRP-oligo at greater than 80% purity.

This kit is an upgraded and improved replacement kit with slightly different components and workflow as the first-generation kit (Cat# CM53401). This kit has multiple configurations for various lengths of oligos and can be used to conjugate 1-5 nano-mole (nmol) of oligo.

• Requirement for amine-oligo:
Amount: 1-5 nano-mole (nmol) oligo content as measured by UV
HPLC purified and lyophilized oligo with amine-oligo content ≥90% by C18 HPLC
≥5- bases (oligos longer than 60 bases may result in lower loading and high impurities).

• Key Features of this HRP-Oligo Conjugation Kit:
High quality maleimide-activated HRP for the conjugation: >99% purity and >200 units/mg protein activity.
A single purification step affords 85~90% of single labeled HRP-oligo conjugates if the quality of the oligo is at par.
Preparation can be done in a day.
Stable linkage with optimal spacer to make sure there is no interference between oligo binding and HRP activation.
All reagents included, from preparation to purification.

Please contact us at for specific academic pricing.

More Details