HRP Antibody Conjugation Kit

The kit is designed to work with any IgG type antibody. The customer supplies their own unmodified IgG and using the kit components converts their antibody to a thiol-antibody followed by reaction of the thiol-antibody with activated HRP to generate the HRP-antibody conjugates. The combination of filtration and scavenger type purification steps typically provides the resulting HRP-antibody at greater than 90% purity.

This kit provides materials to label one (CM51406x1) or three (CM51406x3) antibody samples.

Total amount of activated HRP included in one reaction: 2 mg.

If you have a smaller amount of IgG, non-IgG type antibody, or other customization needs, please select the customization radio button and/or fill out the information above.

• Requirement for Antibody:
IgG type
Amount: 1 mg per reaction
Preferably >90% pure by gel electrophoresis or SEC HPLC analysis

• Key Features of this HRP-Antibody Conjugation Kit:
High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity
Offers a convenient way to prepare HRP−antibody conjugate with heterobifunctional crosslinking reagents
Better control over the conjugation process
Limit self-coupling and polymerization that often encountered when EDC or homobifunctional crosslinkers are used
Target average 1-3 HRP per antibody for a typical batch
Free of or less than 10% unreacted HRP and antibody
All reagents included, from preparation to purification

Please contact us at for specific academic pricing.