BSA-Peptide Conjugation Kit

Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. BSA is often used as a carrier protein for immunization. This personalized BSA conjugation kit is designed to work with any peptide containing a reactive Cys group. The user supplies their own peptide. Then, using the kit components, prepares the BSA-peptide conjugate by reacting their peptide (customer supplied) with activated BSA. A one-step purification process typically provides the resulting BSA-peptide conjugate at greater than 95% purity.

This kit provides materials to perform BSA labeling of one (CM52402x1) or three (CM52402x3) peptides. Total amount of activated BSA included in a reaction: 2 mg.

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• Requirement for Cys peptide:
Amount: 0.15–0.6 µmol (5–20 equivalents of peptide over BSA)
HPLC purified and lyophilized. Please make sure there are no reducing reagents, such as DTT are present.
The Cys peptide should be stored at -80°C.
HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide

• Key Features of this BSA-Peptide Conjugation Kit:
High quality maleimide activated BSA for the conjugation: >95% purity
Optimal maleimide groups per BSA for peptide loading: 20–40 (please check the COA of each individual batch for the exact loading)
A single-step purification affords over 95% BSA-peptide conjugates
Fast preparation: less than 1 hr of hands-on time
All reagents included from preparation to purification

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