HisSep Ni-NTA 6FF Chromatography Column, 1 mL

HisSep Ni-NTA 6FF Chromatography Column, 1 mL

Catalog Number:
ACC1505778YEA
Mfr. No.:
20505ES03/08
Price:
$179
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      • Overview
        • HisSep Ni-NTA Agarose Resin 6FF uses highly cross-linked 6% agarose gel as the matrix, and chemically couples tetracoordinated nitrilotriacetic acid (NTA) as the ligand. After chelating nickel ions (Ni2+), a very stable octahedral structure is formed, and the nickel ions are at the center of the octahedron. This structure can protect nickel ions from the attack of small molecules, is more stable, and can withstand harsh conditions such as a certain concentration of reducing agents, denaturants or coupling agents. In addition, due to the pressure resistance of its matrix (the matrix can withstand a pressure of up to 0.3 MPa), this product can be used for industrial large-scale protein purification, and can achieve purification of target proteins at a relatively high flow rate.

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      • Properties
        • Other Properties
          Matrix: highly cross-linked 6% agarose gel
          Bead size: 45-165 µm
          Capacity: >40 mg 6×His-tagged protein/mL matrix
          Tolerance Pressuremax: 0.3 Mpa, 3 bar
          Buffer: 1×PBS containing 20% ​​ethanol
          Column Size: 0.7×2.5 cm (1mL)
          Storage
          Store at 4°C. Valid for 2 years.
          Shipping
          Shipping with ice packs.
          Note
          It is recommended that the buffer and protein solution used for purification be filtered through a 0.22 µm or 0.45 µm filter membrane before loading onto the column.
          For your safety and health, please operate with lab coats and disposable gloves.
      • Applications
        • Application Description
          HisSep Ni-NTA 6FF Chromatography Column is a medium-pressure prepacked column filled with HisSep Ni-NTA Agarose Resin 6FF. The prepacked column has a standard interface and can be adapted to various commercial medium-pressure chromatography systems, such as ÄKTA, making it convenient for customers to purify His-tag proteins.

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