MBPSep Dextrin 6FF Chromatography Column, 1 mL

MBPSep Dextrin 6FF Chromatography Column, 1 mL

Catalog Number:
ACC1505781YEA
Mfr. No.:
20516ES03/08
Price:
$193
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      • Overview
        • MBPSep Dextrin Agarose Resin is an affinity chromatography medium for purifying proteins tagged with maltose binding protein (MBP). MBP promotes the correct folding of conjugated proteins and increases the solubility of fusion proteins overexpressed in bacteria, especially eukaryotic proteins. MBPSep Dextrin Agarose Resin 6FF can purify MBP fusion proteins in one step. The bound fusion proteins can be gently eluted with 10 mM maltose, which protects the activity of the target protein. The MBP fusion part can be subsequently removed by site-specific proteases. In addition, MBPSep Dextrin Agarose Resin 6FF uses highly cross-linked 6% agarose gel as a matrix, has higher physicochemical properties, can withstand higher pressures, and can purify the target protein at relatively high flow rates, making it more suitable for industrial large-scale protein purification.

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      • Properties
        • Other Properties
          Matrix: highly cross-linked 6% agarose microspheres
          Ligand: dextrin
          Bead size: 45-165 µm
          Capacity: >10 mg MBP protein (80 kDa)/mL matrix
          PressureMax: 0.3 MPa, 3 bar
          pH range: 3-12
          Buffer: 1×PBS containing 20% ​​ethanol
          Storage
          Store at 2~8°C, valid for 2 years.
      • Applications
        • Application Description
          MBPSep Dextrin 6FF Chromatography Column, 1 mL is a medium-pressure prepacked column filled with MBPSep Dextrin Agarose Resin 6FF. The size is 1 mL. The prepacked column has a standard interface and can be adapted to various commercial medium-pressure chromatography systems, such as ÄKTA, etc., which is convenient for customers to operate.

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