HiPure Blood DNA Mini Kit

HiPure Blood DNA Mini Kit

Catalog Number:
NAPK357152ANG
Mfr. No.:
D311102; D311103
Price:
$199
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      • Overview
        • This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

          Principle
          This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

          Advantages
          • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
          • Fast - without separation of leukocytes, organic extraction or ethanol precipitation
          • Simple - all nucleic acids can be obtained by direct digestion
          • Wide applicability- handle a variety of liquid samples

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Purification method: Mini spin column
          Purification technology: Silica technology
          Process method: Manual (centrifugation or vacuum)
          Sample type: Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
          Sample amount: 10-250ul
          Storage
          Proteinase K should be stored at 2–8°Cupon arrival. However, short-term storage (up to 12 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components canbe stored at room temperature (15–25°C) and are stable for at least 18 monthsunder these conditions.

          * For Research Use Only

      • Applications
        • Application
          PCR, southern bolt and virus detection, etc
          Application Description
          Isolation total DNA from 200ul Whole Blood

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