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Overview
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This kit is suitable for high-throughput extraction of total RNA from cultured cells, cell suspensions, animal tissues, conventional fungi and plant tissues. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 60 minutes. The obtained RNA can be directly used in RT-PCR, fluorescence quantification, second-generation sequencing and viral RNA detection and so on. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 hole oscillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
• High purity - OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
• Economy - less than 50% of the price of Qiagen and other imported products
• Automatic - extraction without manual participation, saving time and effort
• Universal - suitable for various clinical samplesPlease contact us at for specific academic pricing.
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- Properties
- Applications
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Overview