SolPure Tissue DNA Kit

This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.

Principle
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.

Advantages
• Safe - without phenol chloroform extraction
• Environment friendly - the reagents used are safe, non-toxic and without pollution
• High molecular weight - the molecular weight of genomic DNA is about 50-150kb
• High purity - the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
• Unlimited sample size - solution type operation, sample volume can be adjusted at will
• Cost performance - the most economical nucleic acid extraction technology

Please contact us at for specific academic pricing.