GST Bestarose 4FF - Tag Affinity Resins

GST Bestarose 4FF - Tag Affinity Resins

Catalog Number:
CM01502172BES
Mfr. No.:
AA0071-AA109314
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      • Overview
        • GST Bestarose 4FF is made by coupling glutathione to a highly cross-linked agarose, which is used to specifically purify glutathione S-Transferase (GST) as well as the purification of GST fusion protein. GST tag is a tag commonly used in modern genetic engineering to express fusion proteins, allowing soluble expression and activity maintenance of proteins. For different sources of glutathione S-transferase and its fusion proteins, High-purity target protein can be obtained in one-step purification when using this resin. The c resin has high pressure resistance, fast flow velocity, and mild operating conditions, which can help with maintenance of protein activity.

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      • Properties
        • Categories
          Tag Affinity Resins
          Other Properties
          Appearance: White slurry
          Matrix: Highly cross-linked agarose, 4%
          Particle size+: 45-165μm
          Functional group: Glutathione with 10 atomic arms
          Ligand concentration: 120~320μmol Glutathione /mL resin
          Dynamic binding capacity: ~10mg GST/mL packed resin
          Max. pressure: 0.3MPa
          Chemical Stability: Stable in common aqueous buffers: 1M HAc++, 70%ethanol, 6M GuHCl (1 hour at room temperature)
          pH stability: 3~12
          Storage+++: 2~30°C, 20%ethanol or 2% benzyl alcohol
          Pressure flow velocity: ~450cm/h, BXK16/10,H=5cm,25°C
          Recommended flow velocity: Sample loading flow velocity: <100cm/h; Equilibration, cleaning, elution flow velocity: 100~300cm/h

          +Particle size is normally distributed, and particles within this range account for more than 95% of the total.
          ++1M HAc only be used for cleaning.
          +++2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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