Name: IMAC Bestarose FF - Tag Affinity Resins
Price: Inquiry

- Overview
  - IMAC Bestarose FF (Fast flow) is a metal chelating affinity chromatography resin, which can be widely used to separate and purify proteins and peptides. The principle is that the side chains of histidine, cysteine and tryptophan of protein interact with a variety of transition metal ions such as Cu2+, Zn2+, Ni2+, Co2+, Fe3+, so as to achieve the purpose of separation and purification.
    IMAC Bestarose FF is crosslinked by cross-linked agarose and aminotriacetic acid (NTA). It can chelate the four price points of metal ions, making the chelated metal ions more stable, to tolerate higher reducing agents, have good physical and chemical stability, as well as advantages in good specificity and fast flow velocity.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Tag Affinity Resins
    
    Other Properties
    
    Appearance: White slurry
    Matrix: Cross-linked agarose, 6%
    Particle size +: 45-165μm
    Metal chelating capacity: Ni2+/ Zn2+: 15μmol/mL resin, Cu2+: 25μmol/mL resin
    Dynamic binding capacity: Ni2+: ~ 40mg His tag protein/mL packed resin
    Chemical stability ++: 1 week at 40°C: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl, 40°C 12h: 1M NaOH, 70% acetic acid.
    pH stability+++: 3~12 (working),2~14 (CIP)
    Pressure flow velocity: ~600cm/h (0.1MPa BXK16/20, H=5cm, 25°C)
    Max. pressure: 0.3MPa
    Storage++++: 2~30°C, 20% ethanol or 2% benzyl alcohol
    Recommended flow velocity: ＜150cm/h
    
    + The particle size is normally distributed, and the particles in this range account for more than 95% of the total.
    ++ Stability when removing metal ions.
    +++ CIP refers to pH stability when metal ions are removed.
    ++++2% benzyl alcohol is only used for international transport or special requirements from customer.
    
    * For Research Use Only

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IMAC Bestarose FF - Tag Affinity Resins

IMAC Bestarose FF - Tag Affinity Resins

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