Benzamidine Bestarose 6B - Affinity Resins

Benzamidine Bestarose 6B - Affinity Resins

Catalog Number:
CM01502175BES
Mfr. No.:
AA511305-AA511314
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      • Overview
        • Benzamidine Bestarose 6B is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 6B agarose resin. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (including trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for the purification of such substances.

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      • Properties
        • Categories
          Affinity Resins
          Other Properties
          Appearance: White slurry
          Matrix: Cross-linked agarose, 6%
          Particle size+: 45~165μm
          Functional group: P-aminobenzidine
          Ligand concentration: ~7μmol P-aminobenzidine /mL resin
          Dynamic binding capacity++: ≥10mg Trypsin/mL packed resin
          Operation flow velocity: ~75cm/h (BXK16/10,H=5cm,25°C)
          Chemical stability: Stable in common aqueous buffers: 6M GuHCl, 8M Urea, PH =1, 2, 3, 4 hydrochloric acid solution, 0.025M borax solution with pH=8, 9, 10, 11
          pH stability: 3~11 (working),2~13 (CIP)
          Storage+++: 2~8°C,20% ethanol + 50mM NaAc (pH4.0) or 2% benzyl alcohol +50mM NaAc (pH 4.0)

          +Particle size is normally distributed, and particles within this range account for more than 95% of the total.
          ++Binding capacity is tested at 50mM Tris + 0.5M NaCl pH8.0.
          +++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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