Name: Ni Bestarose FF - Tag Affinity Resins
Price: Inquiry

- Overview
  - Ni Bestarose FF (Fast Flow) metal chelation chromatography resin is an affinity chromatography resin that pre-chelates metal ion Ni2+ on agarose gel with NTA as ligand. It has the advantages of large adsorption capacity, good selection type, easy regeneration, and low cost. It is widely used in the separation and purification of downstream proteins and peptides in biopharmaceutical and bioengineering, especially the efficient purification of histidine-tagged proteins.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Tag Affinity Resins
    
    Other Properties
    
    Appearance; Turquoise slurry, can be layered
    Matrix; Highly Cross-linked agarose, 6%
    Particle size+; 45~165μm
    Ligand concentration; 12~18μmol Ligand/mL resin
    Dynamic binding capacity; ~ 40mg His tag protein/ mL packed resin
    Chemical stability ++; 40°C One week : 10mM HCl, 0.1M NaOH, 6M GuHCl,8M Urea. 40°C 12h: 1M NaOH, 70% acetic acid.
    pH stability+++; 3~12 (working) 2~14 (CIP)
    Pressure flow velocity; ~600cm/h,BXK16/20,H=5cm,25°C
    Max. pressure; 0.3MPa
    Storage++++; 2~30°C, 20% ethanol or 2% benzyl alcohol
    Recommend flow velocity; ＜150cm/h
    
    +Particle size is normally distributed, and particles within this range account for more than 95% of the total.
    ++ Stability when removing metal ions.
    +++ CIP refers to pH stability when metal ions are removed.
    ++++2% benzyl alcohol is only used for international transport or special requirements from customer.
    
    * For Research Use Only

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Ni Bestarose FF - Tag Affinity Resins

Ni Bestarose FF - Tag Affinity Resins

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