GST Bestarose 4B - Tag Affinity Resins

GST Bestarose 4B - Tag Affinity Resins

Catalog Number:
CM01502171BES
Mfr. No.:
AA009305-AA009314
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      • Overview
        • GST Bestarose 4B is an affinity resin made by coupling glutathione to agarose, which is used to specifically purify glutathione S-transferase (GST) and GST fusion protein. GST tag is a tag commonly used in genetic engineering to express fusion proteins, which facilitates soluble expression and activity maintenance of proteins. For different sources of glutathione S-transferase and its fusion protein, this resin can be used to obtain high-purity target protein in one step of purification. The chromatographic resin has mild operating conditions and helps the maintenance of protein activity.

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      • Properties
        • Categories
          Tag Affinity Resins
          Other Properties
          Appearance: White slurry
          Matrix: 4% agarose
          Particle size+: 45~165μm
          Functional group: Glutathione with 10 atomic arms
          Dynamic binding capacity: >5mg GST/mL packed resin
          Max. pressure: 0.16 bar
          Operation flow velocity: ~75cm/h (BXP16/30 column, H=5cm,25°C)
          Chemical stability: Stable in common aqueous buffers: 1M HAc++, 0.1M NaOH, 70% ethanol, 6M GuHCl (room temperature for 2 hours)
          pH stability: 4~13
          Operating temperature: 2~30°C, do not freeze
          Storage+++: 2~30°C,20% ethanol or 2% benzyl alcohol

          +Particle size is normally distributed, and particles within this range account for more than 95% of the total.
          ++ 1M HAc only be used for cleaning.
          +++2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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