Name: GM-CSF (human) (Recombinant) (His)
Price: Inquiry

- Overview
  - Specific Human GM-CSF is a 24 kDa glycoprotein produced by a variety of cell types, including T and B lymphocytes, macrophages, keratinocytes, eosinophils, neutrophils, and endothelial cells in response to cytokine or inflammatory stimuli. GM-CSF was initially identified on the basis of its capacity to stimulate the clonal proliferation of myeloid precursors in vitro. GM-CSF promotes a Th1 biased immune response, allergic inflammation, and the development of autoimmunity. GM-CSF are particularly effective as antitumor vaccines. GM-CSF regulates multiple biological activities through activation of the GM-CSF receptor (GMCSFR), a member of the type I cytokine receptor group comprising alpha and beta subunits.
    
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- Properties
  - Protein Name
    
    CSF; CSF2; GMCSF; GM-CSF; Molgramostin; Sargramostim; Colony-stimulating Factor; Granulocyte-macrophage Colony-stimulating Factor
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human
    
    Purification
    
    >=95% (SDS-PAGE)
    
    Formulation
    
    Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml. Add 1X PBS to the desired protein concentration. Lyophilized from 0.2µm-filtered solution in 50mM Tris-HCl, 200mM NaCl, pH8.0.
    
    Storage
    
    Short Term Storage +4°C ; Long Term Storage-20°C ; Avoid freeze/thaw cycles. PBS containing at least 0. 1% BSA should be used for further dilutions. Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
    
    Endotoxin
    
    <0.1EU/µg protein (LAL test; Lonza).
    
    Activity
    
    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application
    
    Functional Assay

GM-CSF (human) (Recombinant) (His)

GM-CSF (human) (Recombinant) (His)

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