EZ Cap™ Firefly Luciferase mRNA

Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability.

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Background

EZ Cap™ Firefly Luciferase mRNA will express luciferase protein once entering cells, which is initially extracted from firefly Photinus pyralis. This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc.
EZ Cap™ Firefly Luciferase mRNA is provided at a concentration of 1mg/ml. It is co-transcriptional capped by EZ Cap™ Reagent AG (3' OMe) (Catalog No.B8178) which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). EZ Cap™ Reagent AG (3' OMe) obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with EZ Cap™ Reagent AG. The addition of poly (A) tail increases the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
All the modifications are intention to mimics a fully processed mature mRNA. EZ Cap™ Firefly Luciferase mRNA is an ideal product to observing mRNA delivery, translation and other behaviors.