Erythromycin Estolate

- Overview
  - Erythromycin Estolate, a derivative of Erythromycin, is the lauryl sulfate salt of the proprionic ester of Erythromycin. It acts as a bacteriostatic agent, and has a role as an enzyme inhibitor. Erythromycin Estolate is freely soluble in organic solvents but practically insoluble in water.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Macrolide antibiotics inhibit bacterial growth by targeting the 50S ribosomal subunit preventing peptide bond formation and translocation during protein synthesis. Resistance to Erythromycin is commonly attributed to mutations in 50S rRNA preventing Erythromycin binding allowing the cell to synthesize proteins free of error.
- Properties
  - CAS Number
    
    3521-62-8
    
    Molecular Formula
    
    C40H71NO14 · C12H26SO4
    
    Molecular Weight
    
    1056.39 g/mol
    
    Appearance
    
    White crystalline powder
    
    Solubility
    
    Freely soluble in organic solvents (ethanol, acetone, chloroform, polyethylene glycol, and benzene). Practically insoluble in water.
    
    Other Properties
    
    Source: Actinomycete saccha
    Water Content (Karl Fischer): <4.0%
    Assay: Not less than 610 u/mg (dried basis)
    Melting Point: 132-138 °C
    Identification: Passes test
    
    Storage
    
    ≤30°C. Protect from light
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Erythromycin is a broad-spectrum antibiotic commonly targeting targeting Gram-negative and Gram-positive bacteria. It is also effective against Mycoplasmas including Mycoplasma pneumoniae.
    
    Microbiology Applications: Erythromycin Estolate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive, Gram-negative, and Mycoplasmas species. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include: Mycoplasma pneumoniae 0.0019 µg/mL – 0.0078 µg/mL Legionella pneumophila 0.008 µg/mL – 1 µg/mL Eukaryotic Cell Culture Applications: Cytotoxicity of of Erythromycin Estolate was compared to other macrolides in cultured human non-malignant Chang liver cells and was found the most toxic when compared to Erythromycin, erythromycin-11,12-cyclic carbonate, roxithromycin, clarithromycin and azithromyciin (Viluksel et al, 1996).Gene expression microarrays can prioritize lead compounds in drug development research. In vitro primary rat hepatocytes were exposed to 11 known hepatotoxicants in low-density DNA microarrays with 59 genes known as toxic and metabolic markers for transcription profiling. Erythromycin Estolate was found to cluster in the group with drugs that induced cholestatis. Gene expression data correlated well with the literature (de Longueville et al, 2003).