EasyProbe™ Hoechst 33342 Live Cell Stain

- Overview
  - EasyProbes™ Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence upon binding to the minor groove of dsDNA. Our EasyProbes™ Hoechst 33342 Live Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Hoechst 33342 is widely used for nuclear counterstaining, cell counting, apoptosis and cell cycle studies.
    
    Features
    No need to dilute, weigh, or pipette
    Convenient dropper bottle—just use two drops per mL
    Excited by UV light and emits blue fluorescence at 460 nm when bound to DNA
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    EasyProbe™ Cell Structure Probes
- Properties
  - Categories
    
    Nuclei stains
    
    Other Properties
    
    Excitation/Emission (nm): 350/461
    
    Storage
    
    4°C, protect from light
    
    Shipping
    
    Ambient
    
    * For research use only
- Applications
  - Application
    
    Live-cell stain; Chromosome and nuclear counterstain; Cell-cycle studies
    
    Application Description
    
    EasyProbes™ Hoechst 33342 Live Cell Stain may be added directly to cells in full media, or buffer solutions.
    In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained. In most cases, staining intensity increases with time if cells are not washed prior to imaging.
    EasyProbes™ Hoechst 33342 Live Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter set.
- Reference
  - A plasmid-based reporter system for live cell imaging of dengue virus infected cells.
    Medin CL, Valois S, Patkar CG, Rothman AL,
    J Virol Methods (2015) 211:55-62
    
    Centrifugo-magnetophoretic Purification of CD4+ Cells from Whole Blood toward Future HIV/AIDS Point-of-Care Applications.
    Glynn M, Kirby D, Chung D, Kinahan DJ, Kijanka G, Ducrée J,
    J Lab Autom (2013) –
    
    Complex determinants in specific members of the mannose receptor family govern collagen endocytosis.
    Jürgensen HJ, Johansson K, Madsen DH, Porse A, Melander MC, Sørensen KR, Nielsen C, Bugge TH, Behrendt N, Engelholm LH,
    J Biol Chem (2014) 289:7935-7947

MSDS

Documents

Protocol-FP027-1

COA-FP027

EasyProbe™ Hoechst 33342 Live Cell Stain

EasyProbe™ Hoechst 33342 Live Cell Stain

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Documents

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