EasyProbe™ Green 488 Dead Cell Stain

- Overview
  - EasyProbes™ Green 488 Dead Cell Stain is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA. Our EasyProbes™ Green 488 Dead Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. EasyProbes™ Green 488 Dead Cell Stain is a useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays. It is also suitable for staining nuclei in fixed cell preparations and tissue sections.
    
    Features
    No need to dilute, weigh, or pipette
    Convenient dropper bottle—just use two drops per mL
    Rapid staining of damaged, dead, or fixed cells without wash steps
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    EasyProbe™ Cell Structure Probes
- Properties
  - Categories
    
    Nuclei stains
    
    Other Properties
    
    Excitation/Emission (nm): 500/530
    
    Storage
    
    4°C, protect from light
    
    Shipping
    
    Ambient
    
    * For research use only
- Applications
  - Application
    
    Dead-cell stain; Chromosome and nuclear counterstain
    
    Application Description
    
    EasyProbes™ Green 488 Dead Cell Stain may be added directly to cells in full media or buffer solutions. It easily stains nuclei of fixed cells and tissue slices
    In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
    EasyProbes™ Green 488 Dead Cell Stain is excited with a maximum at 500 nm when bound to DNA, with an emission maximum at 530 nm. It is detected through standard GFP and FITC filters.
- Reference
  - Preclinical pharmacology of AZD5363, an inhibitor of AKT: pharmacodynamics, antitumor activity, and correlation of monotherapy activity with genetic background.
    Davies BR, Greenwood H, Dudley P, Crafter C, Yu DH, Zhang J, Li J, Gao B, Ji Q, Maynard J, Ricketts SA, Cross D, Cosulich S, Chresta CC, Page K, Yates J, Lane C, Watson R, Luke R, Ogilvie D, Pass M,
    Mol Cancer Ther (2012) 11:873-887
    
    The G1 cyclin Cln3p controls vacuolar biogenesis in Saccharomyces cerevisiae.
    Han BK, Aramayo R, Polymenis M
    Genetics (2003) 165:467-476
    
    Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR.
    Hodson JA, Bailis JM, Forsburg SL
    Nucleic Acids Res (2003) 31:e134-e134

MSDS

Documents

Protocol-FP030

EasyProbe™ Green 488 Dead Cell Stain

EasyProbe™ Green 488 Dead Cell Stain

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