EasyProbe™ Propidium Iodide Dead Cell Stain

- Overview
  - EasyProbes™ Propidium iodide is a commonly used dead-cell stain that emits red fluorescence upon binding to DNA. Our EasyProbes™ Propidium Iodide Dead Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Propidium iodide is membrane-impermeant and excluded from viable cells. It is therefore commonly used for identifying dead cells in a population, and for counting dead cells, measuring apoptosis, and sorting cells based on DNA content.
    
    Features
    No need to dilute, weigh, or pipette
    Convenient dropper bottle—just use two drops per mL
    Rapid staining of dead cells without wash steps
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    EasyProbe™ Cell Structure Probes
- Properties
  - Categories
    
    Nuclei stains
    
    Other Properties
    
    Excitation/Emission (nm): 535/617
    
    Storage
    
    4°C, protect from light
    
    Shipping
    
    Ambient
    
    * For research use only
- Applications
  - Application
    
    Dead-cell stain; Chromosome and nuclear counterstain
    
    Application Description
    
    In most cases 2 drops/1×106 cells and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
    EasyProbes™ Propidium Iodide Dead Cell Stain is excited with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser when bound to DNA, with an emission maximum at 617 nm. Generally, propidium iodide fluorescence is detected in the FL2 channel of flow cytometers.
- Reference
  - Extension of Saccharomyces paradoxus chronological lifespan by retrotransposons in certain media conditions is associated with changes in reactive oxygen species.
    VanHoute D, Maxwell PH,
    Genetics (2014) 198:531-545
    
    Modulation of medium pH by caulobacter crescentus facilitates recovery from uranium-induced growth arrest.
    Park DM, Jiao Y,
    Appl Environ Microbiol (2014) 80:5680-5688
    
    Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy.
    Zavodszky E, Seaman MN, Moreau K, Jimenez-Sanchez M, Breusegem SY, Harbour ME, Rubinsztein DC,
    Nat Commun (2014) 5:3828-3828

MSDS

Documents

Protocol-FP028

COA-FP028

EasyProbe™ Propidium Iodide Dead Cell Stain

EasyProbe™ Propidium Iodide Dead Cell Stain

More Details

Documents

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