EasyProbe™ DAPI Fixed Cell Stain

- Overview
  - EasyProbes™ DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence upon binding to the minor groove of dsDNA. Our EasyProbes™ DAPI Fixed Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. DAPI is widely used for nuclear and chromosome counterstain, showing a distinct banding pattern in chromosomes.
    
    Features
    • No need to dilute, weigh, or pipette
    • Convenient dropper bottle—just use two drops per mL
    • Excited by UV light and emits blue fluorescence at 460 nm when bound to DNA
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    EasyProbe™ Cell Structure Probes
- Properties
  - Categories
    
    Nuclei stains
    
    Other Properties
    
    Excitation/Emission (nm): 358/461
    
    Storage
    
    4°C, protect from light
    
    Shipping
    
    Ambient
    
    * For research use only
- Applications
  - Application
    
    Dead-cell stain; Chromosome and nuclear counterstain; Mycoplasma detection
    
    Application Description
    
    In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
    EasyProbes™ DAPI Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter set.
- Reference
  - 6-Phosphogluconate dehydrogenase regulates tumor cell migration in vitro by regulating receptor tyrosine kinase c-Met.
    Chan B, VanderLaan PA, Sukhatme VP,
    Biochem Biophys Res Commun (2013) 439:247-251
    
    A plasmid-based reporter system for live cell imaging of dengue virus infected cells.
    Medin CL, Valois S, Patkar CG, Rothman AL,
    J Virol Methods (2015) 211:55-62
    
    An iron-regulated and glycosylation-dependent proteasomal degradation pathway for the plasma membrane metal transporter ZIP14.
    Zhao N, Zhang AS, Worthen C, Knutson MD, Enns CA,
    Proc Natl Acad Sci U S A (2014) -111(25):9175-80.

MSDS

Documents

Protocol-FP026

COA-FP026

EasyProbe™ DAPI Fixed Cell Stain

EasyProbe™ DAPI Fixed Cell Stain

More Details

Documents

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