Name: Cyclosporin A, EvoPure®
Price: 187.00 USD

- Overview
  - Cyclosporin A, EvoPure® is a neutral, cyclic oligopeptide with immunosuppressive activity. Cyclosporin A has the most potent immunosuppressive activity of the metabolites (Cyclosporin B, C, D, E, and H). Cyclosporin A has been used to prevent organ transplant rejection. Cyclosporin A is soluble in ethanol and DMSO.
    EvoPure® products have been fully characterized by spectral analysis and are shipped with a comprehensive certificate of analysis containing lot-specific HPLC, MS, HNMR, and FTIR data.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    After entering a T-cell, Cyclosporin A associates with the cytosolic protein cyclophilin which helps in protein folding. Cyclosporin A binds to cyclophilins and this complex binds another cytosolic protein phosphatase called Calcineurin (protein phosphatase 2B) that dephosphorylates a transcription factor (nuclear factor of activated T-cells, or NF-AT) needed for expression of interleukin 2 (IL-2.). It also blocks the pathway to nitric oxide synthesis via tumor necrosis factor (TNFa) and Interleukin 1a.
- Properties
  - CAS Number
    
    59865-13-3
    
    Molecular Formula
    
    C62H111N11O12
    
    Molecular Weight
    
    1202.6 g/mol
    
    Appearance
    
    White powder
    
    Solubility
    
    Soluble in ethanol (50 mM) and DMSO (100 mM)
    
    Other Properties
    
    Source: Tolypocladium inflatum
    Assay: ≥99.0% (HPLC, as is)
    Identification: IR, MS, NMR
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Eukaryotic Cell Culture Applications: Cyclosporins have used as tools to study complex biological networks and pathways, involving protein function, and protein-protein interactions. Cyclosporin A had a suppressive effect on the Hepatitis C virus (HCV) replicon at the RNA level and HCV protein expression in cultured hepatocytes. Cyclosporin A also inhibited multiplication of the HCV genome in a human hepatocyte cell line infected with HCV using HCV-positive plasma (Watashi et al, 2003). Cyclosporin A has been used to establish Epstein-Barr virus-transformed human lymphoblastoid cell lines (Anderson and Gusella 1984). Cyclosporin’s immunosuppressive properties and potential toxicity can be studied during in vitro assays. Other metabolites of Cyclosporin A (AM1, AM1c, DihydroAM1, AM19, and AM4N) can also be studied (Vollenbroeker B et al, 2005).