Cy5 amine (non-sulfonated)

- Overview
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    Background
    
    Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
    In A2780 cells, the conjugates polymer 2P-Cy3-Cy5 can be used for the analysis of the pair-correlation functions between Cy3 and Cy5. Treated with 2P-Cy3-Cy5 for several hours show similar shape and characteristic decay lengths [1].
    
    Reference: [1]Hartley, J. M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59.
- Properties
  - Categories
    
    can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.
    
    Alternative Name
    
    1-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium
    
    Molecular Formula
    
    C38H54Cl2N4O
    
    Molecular Weight
    
    653.77
    
    Appearance
    
    A solid
    
    Purity
    
    98.80%
    
    Solubility
    
    ≥48 mg/mL in DMSO; insoluble in H2O; ≥8.84 mg/mL in EtOH
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    CC1(C)C2=C(C=CC=C2)[N+](CCCCCC(NCCCCCC[NH3+])=O)=C1/C=C/C=C/C=C3C(C)(C)C4=CC=CC=C4N\3C
    
    * For Research Use Only
- Reference
  - 1. Zhang L, Yang P, et al. "Multifunctional Mesoporous Polydopamine With Hydrophobic Paclitaxel For Photoacoustic Imaging-Guided Chemo-Photothermal Synergistic Therapy." Int J Nanomedicine. 2019 Nov 4;14:8647-8663. PMID:31806962
    2. Qin Jiang, Zimiao Luo, et al. "Red blood cell membrane-camouflaged melanin nanoparticles for enhanced photothermal therapy." Biomaterials. S0142-9612(17)30483-0.