Cy3.5 maleimide (non-sulfonated)

- Overview
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    Background
    
    Cy3.5 maleimide can be used for selective labeling of free sulfhydryl groups (e.g. cysteine residues in peptides, proteins and oligonucleotides). Before labeling, cystines should be prepared with reductant such as TCEP (tris-carboxyethylphosphine). The mono-reactive malemides is particularly good for the labeling of antibody, that’s because the dye molecules are located away from the antibody binding site and functioning of the labeled molecules can be protected.
- Properties
  - Categories
    
    Labeling of cysteine residues, as well as other thiolated molecules
    
    Alternative Name
    
    3-(6-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E)-3-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium chloride
    
    Molecular Formula
    
    C44H47ClN4O3
    
    Molecular Weight
    
    715.32
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥71.5 mg/mL in DMSO; ≥28.5 mg/mL in EtOH with ultrasonic
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C4=[N+](CCCCCC(NCCN5C(C=CC5=O)=O)=O)C6=CC=C(C=CC=C7)C7=C6C4(C)C.[Cl-]
    
    * For Research Use Only