Cy3 alkyne (non-sulfonated)

- Overview
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    Background
    
    Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it. The reagent can be efficiently attached to water-soluble molecules such as proteins when used with organic co-solvent (DMSO or DMF). For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. In dendritic cells, the research confirming that the conjugates of Cy3-alkyne and Cy5-BSA-pDMDOMA particles are internalized by the cells rather than sticking to the cell surface. Additionally, confocal microscopy imaging shows a strong co-localization of the Cy3 and Cy5 channel [1].
    
    Reference: [1] Vanparijs, N. ; De Coen, R.; Laplace, D.R.; Louage, B.; Maji, S.; Lybaert, L.; Hoogenboom, R.; De Geest, B. Transiently responsive protein-polymer conjugates via a grafting-from RAFT approach: for intracellular co-delivery of proteins and immune-modulators.Chemical Communications, 2015, 51, 13972–13975.
- Properties
  - Categories
    
    used for Click chemistry to label any molecule bearing azide group.
    
    Alternative Name
    
    6-[(2E)-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethylindol-1-ium-2-yl)prop-2-enylidene]indol-1-yl]-N-prop-2-ynylhexanamide
    
    Molecular Formula
    
    C33H40ClN3O
    
    Molecular Weight
    
    530.14
    
    Appearance
    
    A solid
    
    Purity
    
    98.53%
    
    Solubility
    
    soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
    
    Storage
    
    Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
    
    SMILES
    
    CC1(C2=CC=CC=C2[N+](=C1C=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)NCC#C)(C)C)C)C
    
    * For Research Use Only