Cy5 alkyne (non-sulfonated)

- Overview
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    Background
    
    Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Various substrates such as biomolecules, polymers, and solid surfaces which bearing azides can be used for the labeling. In the azido-GalNAc samples, alkyne-Cy3 and alkyne-Cy5 specifically label azido-proteins. Furthermore, researches also indicate that the Cy5 and Cy3 probe-protein conjugates have non-overlapping fluorescence profiles and can be differentially detected within a single gel [1].
    
    Reference: [1] Burnham-Marusich, A. R.; Plechaty, A.M.; Berninsone, P.M. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis, 2014, 35(18), 2621-2625.
- Properties
  - Categories
    
    used for Click chemistry to label any molecule bearing azide group.
    
    Alternative Name
    
    3,3-dimethyl-1-(6-oxo-6-(prop-2-yn-1-ylamino)hexyl)-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium chloride
    
    Molecular Formula
    
    C35H42ClN3O
    
    Molecular Weight
    
    556.18
    
    Appearance
    
    A solid
    
    Purity
    
    99.10%
    
    Solubility
    
    ≥55.6 mg/mL in DMSO; insoluble in H2O; ≥50 mg/mL in EtOH with ultrasonic
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    CC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(NCC#C)=O)C(C=CC=C4)=C4C3(C)C.[Cl-]
    
    * For Research Use Only