Cy5 carboxylic acid (non-sulfonated)

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Cy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred. This labeling dye has limited water solubility. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In the cells, The Cy5 carboxylic acid label on the dye can track the efficiency of the protein expression [1].
    
    Reference: [1] Deyle, K. M.; Farrow, B.; Hee, Y.Q.; Work, J.; Wong, M.; Lai, B.; Umeda, A.; Millward, S.W.; Nag, A.; Das, S.; Heath, J.R. A protein-targeting strategy used to develop a selective inhibitor of the E17K point mutation in the PH domain of Akt1. Nature Chemistry,2015, 7, 455–462.
- Properties
  - Categories
    
    Non-reactive fluorophore, for control experiments, and for calibration
    
    Alternative Name
    
    6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoic acid
    
    Molecular Formula
    
    C32H39ClN2O2
    
    Molecular Weight
    
    519.12
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥51.9 mg/mL in DMSO; insoluble in H2O; ≥89 mg/mL in EtOH
    
    Storage
    
    24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
    
    SMILES
    
    CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)O)(C)C)C)C
    
    * For Research Use Only