Cells from MOG or PLP-immunized mice - fresh

Cells from MOG or PLP-immunized mice - fresh

Catalog Number:
BC01578976HOO
Mfr. No.:
CMPIM-0110; CMPIM-0111; CMPIM-0112; CMPIM-0113; CMPIM-0114; CMPIM-0115; CMPIM-0116; CMPIM-0117; CMPIM-0118
Price:
$728
  • Size:
    Quantity:
    Add to Cart:
      • Overview
        • Snap frozen mouse tissue from well-defined stages and severity of EAE, and fresh cells from EAE-immunized mice.

          Analyze gene expression and protein presence in EAE tissue without a vivarium
          Save time establishing & running EAE models; most tissue products are available from stock with no lead time.

          Choose individual mice from our inventory based on EAE severity
          Full EAE score history provided for each sample.

          Use fresh cells for adoptive transfer or T cell function analysis.
          Suspensions shipped cold overnight, ready for use.

          Tissue from control and naïve mice available for comparison
          Control tissues are matched for immunization method and timing.

          Female mice of age 8 to 14 weeks are acclimated to our facility for at least 7 days, then immunized with antigen subcutaneously in the lower back, close to the base of tail, as follows:

          11 days later (10 days for SJL mice), mice are euthanized and cell suspension(s) from spleen and/or inguinal lymph nodes are prepared.

          Red blood cells in spleen are lysed.

          Cells are resuspended in complete tissue culture media and cell concentrations determined and marked on tubes.

          Cells are shipped the same day for overnight delivery.

          Please contact us at for specific academic pricing.

      • Properties
        • Other Properties
          Centrifuge tubes (15 or 50 mL) with cells in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).
          Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cells will be pooled in one tube with one cell count.
          Storage
          Fresh cells ship cold on gel packs in insulated packaging. Use or freeze within 24 hours of shipment. For this reason fresh cells ship to US and Canadian addresses only, via FedEx overnight.

          * For Research Use Only.