Bialaphos ReadyMade™ Solution

71048-99-2

C11H21N3O6S2P·Na

345.27

Mechanism of Action: Bialaphos acts as a proherbicide by separating into individual subunits of alanylalanine and phosphinothricin. Phosphinothricin is toxic to cells as it triggers the accumulation of ammonia, thus inhibiting photosynthesis and glutamine synthesis.

-20°C

Spectrum: Bialaphos is a broad-spectrum herbicide that also has activity against Gram-positive and Gram-negative bacteria, and certain fungi.

Microbiology Applications:
Bialaphos Sodium was used to study its effects on oligopeptide transporters in a Sinorhizobium meliloti hfq mutant (Sobrero et al, 2011).
The pat gene from Streptomyces viridochromogenes confers resistance to Bialaphos, and it is often incorporated in yeast genetics. Yeast cells that carry the pat resistance gene can grow, and resistance is a dominant trait. This dominant resistance trait is useful when studying wild yeast strains.

Plant Biology Applications:
Bialaphos is used in transformation of cereals including wheat, rice, maize, barley, sorghum, oat and rye. The bar gene is incorporated in the plant genome along with the gene of interest. When Bialaphos is applied, only plants which are successfully transformed survive. It is more effective than glufosinate. Once inside the plant cell, bialaphos is hydrolyzed and converted to phosphonothricin via the phosphinothricin acetyl transferase enzyme. Phosphinothricin is toxic to plants, causing build up of ammonia, which in turn inhibits glutamine synthetase.