BcMag™ Boronate Affinity Magnetic Beads

Affinity purifications are popular tools for the separation of biological compounds. Among them, boronate affinity chromatography is unique because the ligand (m-aminophenyl boronic acid) specifically and reversibly binds the vicinal diol of cis-diol-containing compounds at pH 8-9 and dissociates at pH 3-4 or addition of sorbitol. The boronate affinity resin is a powerful tool for efficiently enriching cis-diol-containing compounds such as alcohols, nucleosides, nucleotides, nucleic acids, carbohydrates, glycoproteins, and enzymes from complex samples such as human serum. Although current boronate affinity-based different chromatography matrices such as columns are available, their procedures are tedious, time-consuming, and unable to handle very tiny samples such as cancer-cell targeting and single-cell analysis. We developed a novel, efficient magnetic affinity system to overcome these limitations. BcMag™ Boronate Affinity Magnetic Beads are inert silica-enclosed magnetic beads grafted with a high density of borinic acid groups on the surface. The beads work perfectly as solid resin for various affinity purifications of cis-diol-containing compounds. Due to its magnetic property, the magnetic matrix can easily fit into an automation system for high throughput assays.

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