Name: Anti-MK5/MAPKAPK5 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Map Kinase-Activated Protein Kinase 5; Mapk-Activated Protein Kinase 5; Mapkap Kinase 5; Mapkap-K5; Mapkapk-5; Mk-5; Mk5; P38-Regulated/Activated Protein Kinase; Prak (Gene ID:8550; UniProt:Q8IW41)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IP; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/ml.
    IHC-In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8µg/ml.
    IF-Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts retrovirally transduced wiith Mk5/Erk3 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of the IP were labelled with mouse anti-MK5 or with rabbit anti-ERK3 serum. An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

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