Name: Anti-MK5/MAPKAPK5 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Map Kinase-Activated Protein Kinase 5; Mapk-Activated Protein Kinase 5; Mapkap Kinase 5; Mapkap-K5; Mapkapk-5; Mk-5; Mk5; P38-Regulated/Activated Protein Kinase; Prak (Gene ID:8550; UniProt:Q8IW41)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IP; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:64000.
    WB-In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/ml.
    IHC-In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8µg/ml.
    IF-Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts retrovirally transduced wiith MK5/ERK3 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of the IP were labelled with mouse anti-MK5 or with rabbit anti-ERK3 serum. An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

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