Name: Anti-MK2/MAPKAPK2 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Map Kinase-Activated Protein Kinase 2; Mapk-Activated Protein Kinase 2; Mapkap Kinase 2; Mapkap-K2; Mapkapk-2; Mk-2; Mk2 (Gene ID:9261; UniProt:P49137)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IP; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 60kDa band observed in Mouse Embryonic Fibroblasts lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction. The observed molecular weights correspond to earlier findings in literature with different antibodies :1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2µg/ml.
    IHC-Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.
    IF-Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2. An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.