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Overview
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Recognizes a protein of 86 kD on the cell surface and within the cytoplasm of maturemacrophages. The antibody is associated with fully differentiated tissue macrophages both in normaland in diseased tissues, independently of the presence or absence of inflammation. The antigen isabsent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture humanmonocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermorethe monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful formacrophage phenotyping.Immunogen: Human blood monocytes
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