Anti-Luciferase antibody

Anti-Luciferase antibody

Catalog Number:
A001053120STJ
Mfr. No.:
STJ97752
Price:
$224
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      • Overview
        • Luciferase or Luciferin 4-monooxygenase is a protein encoded by the luc gene which is produced in Photinus pyralis or the common eastern firefly. Luciferase is localised to the peroxisome and is involved in chaperone binding. This protein falls under the ATP-dependent AMP-binding enzyme family and is one of the most extensively studied of the enzymes that catalyse light production in bioluminescent organisms and requires ATP and 02 as substrates. There are two reactions catalysed by luciferase, the first being the formation of an enzyme-bound luciferyl-adenylate and a second in which the luciferyl-adenylate undergoes an oxidative decarboxylation which results in the production of CO2, oxyluciferin, AMP, and green light with a wavelength of 562 nm. STJ97752 was developed from clone 6B8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of Luciferase.

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      • Properties
        • Host
          mouse
          Antibody Type
          monoclonal
          Isotype
          IgG1
          Reactivity
          firefly
          Clone
          6B8
          Immunogen
          Recombinant peptide derived from Luciferase
          Purification
          The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
          Conjugate
          Unconjugated
          Formulation
          Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
          Storage
          Store at -20°C and avoid repeat freeze-thaw cycles.
          Concentration
          1 mg/ml
          Regulatory Status
          RUO
          Antigen
          Luciferin 4-monooxygenase; Luciferase (UniProt:P08659)

          * For research use only

      • Applications
        • Application
          IF; WB
        • Application Dilutions
          IF 1:50-200
          WB 1:1000-2000

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