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Overview
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Lamin B1 is a protein encoded by the LMNB1 gene which is approximately 66, 4 kDa. Lamin B1 is localised to the nuclear inner membrane. It is involved in the apoptosis and survival caspase cascade , mitotic cell cycle, granzyme pathway and CDK-mediated phosphorylatin. It is one of the two B-type lamin proteins and is a component of the nuclear lamina. It is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin B1 is expressed in the liver, nervous system, eye, heart and intestine. Mutations in the LMNB1 gene result in autosomal dominant, adult-onset leukodystrophy. STJ96938 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Lamin B1 protein.
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Overview
Western blot analysis of lysates from 1) HepG2, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
1) Input: Mouse Brain Tissue Lysate , 2) IP product: IP dilute 1:200 Western blot analysis of 1) HepG2, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of Human-lung-cancer tissue. 1, Lamin B1 monoclonal antibody (7C11) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Lamin B1 monoclonal antibody (7C11) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1, AMPK Alpha 1/2 (phospho Thr183/172) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-Lamin B1 antibody
Anti-Lamin B1 antibody
Catalog Number:
A001052307STJ
Mfr. No.:
STJ96938
Price:
$224
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