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Overview
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GAPDH is a protein encoded by the GAPDH gene which is approximately 36 kDa. It is localised to the cytoplasm and nucleus where it is involved in glucose metabolism, respiratory electron transport, carbon metabolism and HIF-1 signalling pathway. It is a moonlighting protein based on its ability to perform mechanistically distinct functions, which catalyses an important energy-yielding step in carbohydrate metabolism and also has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions. GAPDH is expressed in the blood, eyes, intestine, kidney and liver. Mutations may result in FMR1-related disorders. STJ96931, monoclonal GAPDH antibody targets endogenous GAPDH, and was developed from clone 2B8.
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