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Overview
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beta I tubulin is a protein encoded by the TUBB1 gene which is approximately 50, 3 kDa. beta I tubulin is localised to the cytoplasm. It is involved in development Slit-Robo signalling, Sertoli-Sertoli cell junction dynamics, signalling in gap junctions and chaperonin-mediated protein folding. This protein falls under the beta tubulin protein family. It is one of two core protein families that heterodimerize and assemble to form microtubules. It is exists in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. beta I tubulin is expressed in the blood and cells of the nervous system. Mutations in the TUBB1 gene may result in macrothrombocytopaenia. STJ96939 was developed from clone 3F7 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous beta I tubulin proteins.
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Overview
Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue, diluted at 1:5000.
Immunofluorescence analysis of Human-breast-cancer tissue. 1, Beta I tubulin monoclonal antibody (3F7) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Beta I tubulin monoclonal antibody (3F7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, Beta I tubulin monoclonal antibody (3F7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, Beta I tubulin monoclonal antibody (3F7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-Beta I tubulin antibody
Anti-Beta I tubulin antibody
Catalog Number:
A001052308STJ
Mfr. No.:
STJ96939
Price:
$224
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