Name: Anti-GAPDH antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Glyceraldehyde-3-Phosphate Dehydrogenase; Gapdh; Peptidyl-Cysteine S-Nitrosylase Gapdh (Gene ID:2597; UniProt:P04406)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3. Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: PMID: 29953622 and PMID: 29158223, and in WB on Rat: PMID: 31087788.
    IHC-In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10µg/ml.