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Overview
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Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
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Overview
Immunofluorescence analysis of HeLa cells using HDAC6 antibody (STJ113047) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (STJ113047). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ113047) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using HDAC6 antibody (STJ113047) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Anti-HDAC6 antibody
Anti-HDAC6 antibody
Catalog Number:
A001020484STJ
Mfr. No.:
STJ113047
Price:
$297
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