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Overview
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The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
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Overview
Immunofluorescence analysis of NIH-3T3 treated by Chloroquine and 293T cells using MAP1LC3B Mouse mAb (STJ119545) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293T cells using MAP1LC3B Mouse mAb (STJ119545) at dilution of 1:100 (40x lens). 293T cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using MAP1LC3B Mouse mAb (STJ119545) at dilution of 1:100 (40x lens).C6 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MAP1LC3B antibody (STJ119545) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37°C for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
Anti-MAP1LC3B antibody
Anti-MAP1LC3B antibody
Catalog Number:
A001026272STJ
Mfr. No.:
STJ119545
Price:
$295
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