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Overview
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Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
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Overview
Confocal immunofluorescence analysis of L929 cells using HDAC1 Polyclonal Antibody (STJ23928) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (STJ23928). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ23928) at a dilition of 1:1000.
Immunofluorescence analysis of A549 cells using HDAC1 antibody (STJ23928). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody (STJ23928) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (STJ23928) at dilution of 1:100 (40x lens).
Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (STJ23928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using HDAC1 antibody (STJ23928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
Anti-HDAC1 antibody
Anti-HDAC1 antibody
Catalog Number:
A001034081STJ
Mfr. No.:
STJ23928
Price:
$295
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